dispersion staining


dispersion staining
   A technique for refractive index determination that employs central or annular stops placed in the objective back focal plane of a microscope. Using an annular stop with the substage iris closed, a fiber mounted in a high dispersion medium will show a colored boundary of a wavelength where the fiber and the medium match in refractive index. Using a central stop, the fiber will show colors complimentary to those seen with an annular stop.

Forensic science glossary. . 2012.

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